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Relaxed dna

relaxed DNA

(Science: molecular biology) dNA that isn't supercoiled.


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Re: problem with cloning/restriction digest

... both replies. Yes. I too believe that the problem lies in the vector DNA to begin with. Yesterday, when I ran my uncut and dbl digested vector ... you are seeing significant amounts of supercoiled and/or relaxed vector in your digests, then your enzyme(s) isn’t cutting very efficiently—or ...

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by ashlie23
Sat Feb 09, 2008 5:02 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 9011

Re: problem with cloning/restriction digest

If you are seeing significant amounts of supercoiled and/or relaxed vector in your digests, then your enzyme(s) isn’t cutting very efficiently—or are you just worried about any residual uncut vector DNA? Is your vector DNA prep really old? Maybe you just need to make a fresh prep ...

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by blcr11
Fri Feb 08, 2008 3:38 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 9011

God vs Evolution

... oneself up than by attributing the unknown solution to God's mystical ways? You then do not have to think about the problem anymore and you feel relaxed again.

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by genovese
Thu Nov 29, 2007 4:03 pm
 
Forum: Evolution
Topic: God vs Evolution
Replies: 266
Views: 26816

ITS-PCR

... important for size than for "meaning" they are likely to have relaxed evolutionary pressure, and are likely to change quickly (relatively), ... RAPD is random amplified polymorphic DNA The method is based on the Polymerase Chain Reaction (PCR) using short ...

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by canalon
Thu Sep 06, 2007 2:14 pm
 
Forum: Molecular Biology
Topic: ITS-PCR
Replies: 2
Views: 883

The need to stretch?

... :) It makes my entire body feel soooooo good and relaxes me. Almost like a full body massage all at once. Just thinkng about it makes me feel relaxed. :)

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by Madeline
Thu Jul 05, 2007 5:20 pm
 
Forum: General Discussion
Topic: The need to stretch?
Replies: 21
Views: 4196
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