Rehydrate

hey, during an experiment we were preparing tissue sample to be stained using H and E. we had to rehydrate the sample before entering it into the dye i found out that the reason for this is to get the tissue to as life like state as possible before staining. is there also ...

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... method of in gel rehydration without making fresh reagents (rehydration buffers). I have a 110mm long IPG strip and I know, that there is a way to rehydrate it with my cell lysate, adding two buffers (I don't know its consistention). Thank you in adwance, Andrius

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... shape like the ones I saw, but there appear to be a number of types. I guess once I have them, my first experiment will be to dehydrate and the rehydrate and see if they come back. That would be pretty compelling evidence. Pete

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