Rci recombinase

... In a Holliday junction, does it cause the crossing over or does it does it cut the strands after it crosses over? And is it the same thing as recombinase? Thanks!

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... It stands for "floxed" which itself stands for "flanked by lox P sites" the lox P sequence being the recognition site for Cre recombinase. See Kuhn, et. al. (1995) Science 269, 1427-1429. You put Cre recombinase under the control of a promoter (Mx1) which is sensitive to stimulation ...

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... while under non-permissive conditions you may see the effect of the absence of the gene’s expression. For the fl/fl cells, in the absence of Cre recombinase, Rb is present, just as if it were a normal cell. When you transfect with something that carries the recombinase, Rb is excised from both ...

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Site specific DNA recombination involves recombinase. Transposase is not specific enough.

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... transfer to any number of pre-designed acceptor vectors for expression in bacterial or eukaryotic or insect cells. Invitrogen’s system uses lambda recombinase to effect the transfer. I think Clontech’s system uses Cre/lox recombinase and a different series of donor/acceptors, but it’s the same ...

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