Puromycin

... by RNA PoII III) has been hypermethylated in LNCaP cells but i doubt that since i used very early passage cells after drug selection (2.0 ug/ml puromycin) for the western blot analysis, so from the point of kinetics that does not make sense. They also mentioned that maybe due to the processing ...

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... -80C until usage. Day 4. Morning: change medium for infected MEFs. Day 5. Split infected MEFs at 1:2 or 1:3 for either NO drug selection or 2ug/ml puromycin selection On Day 3 afternoon, during the 2nd infection, I could see MEFs change their morphology(become more flat) and some MEFs look like ...

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... the stably transfected Phoenix cells in order to produce hight titer supernatants, but the problem is that my retroviral construct do not carry puromycin resistance, only carries GFP so, how can I select for the stably transfected Phoenix cells? Is the retroviral vector integrated into the phoenix ...

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