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Pta stain

PTA stain --> phosphotungstic acid stain

(Science: technique) The first general stain used for electron microscopy; a selective stain for extracellular components such as elastin, collagen, and basement membrane mucopolysaccharides; it can be followed by uranyl acetate or lead.

Synonym: pTA stain.


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separation problem with GelRed

I am a bit surprised here, you say that post satining is too time consuming but you are ready to slow down your gels. It takes 10 minutes for staining, and about as much if you want to destain, which should not even be a problem with gelred. So why not post stain? If this is really not an option, ...

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by canalon
Mon Aug 24, 2009 4:49 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 1
Views: 65

Re: Methods Identifying Unknown Bacteria?

Gram's stain is certainly useful in classification but not always truthful. Some organisms, Acinetobacter in particular, can readily stain gram-positive in fresh clinical material, yet stains gram-negative after growth on plated ...

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by stochastic
Fri Jul 24, 2009 2:00 am
 
Forum: Microbiology
Topic: Methods Identifying Unknown Bacteria?
Replies: 5
Views: 226

Methods Identifying Unknown Bacteria?

Isn't the first thing to do is a Gram stain? And then find out if it is anaerobic, aerobic, or facultative? then look at it under a microscope for its shape? what about the media that it can grow on? put different antibiotics in the media and ...

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by kolean
Wed Jul 22, 2009 12:42 pm
 
Forum: Microbiology
Topic: Methods Identifying Unknown Bacteria?
Replies: 5
Views: 226

i need this article please help me

well i'm doing an work with imageJ software with colour deconvolution (detection of DAB stain) and i need for validity this article: Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol 23: 291-299, 2001 if someone has this article ...

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by GVB
Sun Jul 12, 2009 8:12 pm
 
Forum: General Discussion
Topic: i need this article please help me
Replies: 0
Views: 80

Some problems of DNA recovery from native PAGE gel

... recovery is as follows, 1. 20% native PAGE gel (The DNA i want to purify is around 20bp dsDNA + 10 bp ssDNA ) 2. running at 250 v for 30mins, 3. stain and cut the desired band; 4. cursh the gel by yellow tips into small gains; 5. add 2volume TE buffer (100ug gel ~ 200ul TE buffer)., rotating ...

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by wusilinqi
Thu May 14, 2009 5:20 am
 
Forum: Genetics
Topic: Some problems of DNA recovery from native PAGE gel
Replies: 1
Views: 546
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