Proofreading

... the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. I'd expect more bands not ...

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... where data is being copied onto mRNA), whereas replication is started from the origin of replication (i.e. ori) site(s), which is where the proofreading activity is crucial. I must admit I'm bit confused about the original question, as I'm not sure if it means gene expression or DNA replication; ...

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... ! But my guess for reason #2 would be for accuracy reasons in replicating the DNA in multiple short strands with the DNA repair and DNA accuracy proofreading complexes could do a proficient job. I am also coming up with a reason of chromatin modeling lately. As the DNA is being replicated, chromatin ...

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... but who knows...) actually try to understand it rather than just dealing with a 2 lines quote. - I know, we all make typos but a minimum amount a proofreading would at least prove that you actually care about what you are talking about. 1/ I do not see why the Cambrian explosion would invalidate ...

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... by mutations is a different story: it can happen at any stage of the cells' life, especially if we are talking about dividing cells. Unless the proofreading mechanisms and error repair can fix the genome, and the mutation is "fatal" for the cell, it dies either because of failed mitosis, ...

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