Probe-pointed

... pulling moment here i cant determine what my northern blot results mean i hope someone can help... i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does ...

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... pulling moment here i cant determine what my northern blot results mean i hope someone can help... i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does ...

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Can anyone help me here Iam struggling to understand how to calculate the amount of DNA probe i need for my hybridization solution in a blot. say for a hybridization solution of 450 microliter, I have 0.5pmol DNA probe, what volume of the probe i need for this hybridization ...

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Hi guys, I'm preparing the probe for in situ hybridization. It's the product from degenerated PCR so i guess it's ssDNA. I labeled the probe with DIG, keep in 50%formamide and hybridization buffer with carrier DNA. I was so stupid that ...

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... i. Cellular DNA is treated with EcoRI ii. The DNA then is treated with gel electrophoresis and then moved to membrane nitrocellulose. iii. DNA ras probe with radioactive labels then was applied to the x-ray film iv. The autoradiograph result : (see image) PIC344.jpg 1. leucocyte from a healthy ...

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