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Phosphatase

phosphatase

(Science: enzyme) That hydrolyse phosphomonoesters. Acid phosphatases are specific for the single charged phosphate group and alkaline phosphatases for the double charged group. These specificities do not overlap.

The phosphatases comprise a very wide range of enzymes including broad and narrow specificity members. Phosphoprotein phosphatases specifically de phosphorylate a particular protein and are essential if phosphorylation is to be used as a reversible control system. Any of a group of enzymes that act as a catalyst in the hydrolysis of organic phosphates.A type of [[enzyme capable of releasing a [[phosphate from its parent molecule, e.g. the release of phosphate from [[ATP to release energy and the phosphate.


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cdc25 and tyrosin-15

I was given the following question: The cdc25 gene of the fission yeast Saccharomyces pombe encodes a phosphatase which dephosphorylates the tyrosine 15 residue of the cyclin-dependent kinase (Cdk). Dephosphorylation of tyrosine-15 of Cdk activates its kinase activity. You ...

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by jb0378
Wed Jul 29, 2009 10:18 pm
 
Forum: Cell Biology
Topic: cdc25 and tyrosin-15
Replies: 1
Views: 96

DNA ligases

No. It'll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.

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by Sepals
Fri Mar 27, 2009 11:44 am
 
Forum: Molecular Biology
Topic: DNA ligases
Replies: 1
Views: 434

pcDNA 4/TO

... I can do to get the insert to be taken up? Or is there something I am doing wrong? Note: I have also already done treatment of shrimp alkaline phosphatase on the vectors to remove the phosphates and lessen the occurrence of self-ligation

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by am1795
Mon Jan 12, 2009 6:58 pm
 
Forum: Molecular Biology
Topic: pcDNA 4/TO
Replies: 1
Views: 458

Converting Glucose to Fat or ATP?

... used when blood glucose is low. The antagonistic effect of insulin, produced when blood glucose is high, is attributed to activation of Protein Phosphatase." You need to keep in mind what the effects of insulin are.

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by MrMistery
Sun Dec 14, 2008 3:48 pm
 
Forum: Cell Biology
Topic: Converting Glucose to Fat or ATP?
Replies: 12
Views: 1837

I have a question on glucose here!

i think the same phosphoisomerase will first convert G1P to G6P and then the almighty glucose 6-phosphatase will cut the phosphate, yielding free glucose. Also, glucagon doesn't trigger glycogenolysis in muscle, only in the liver. therefore the most important is the pathway ...

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by MrMistery
Fri Jul 04, 2008 9:33 pm
 
Forum: Human Biology
Topic: I have a question on glucose here!
Replies: 8
Views: 1475
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