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Phenol

phenol

(Science: chemistry)

1. A white or pinkish crystalline substance, C6H5OH, produced by the destructive distillation of many organic bodies, as wood, coal, etc, and obtained from the heavy oil from coal tar.

It has a peculiar odour, somewhat resembling creosote, which is a complex mixture of phenol derivatives. It is of the type of alcohols, and is called also phenyl alcohol, but has acid properties, and hence is popularly called carbolic acid, and was formerly called phenic acid. It is a powerful caustic poison, and in dilute solution has been used as an antiseptic.

2. Any one of the series of hydroxyl derivatives of which phenol proper is the type.

(Science: chemistry) glacial phenol, any one of a series of compounds having both phenol and aldehyde properties. Phenol phthalein. See Phthalein.

Origin: Gr. To show _ -ol: cf. F. Phenol.


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RNA from feathers

... and I am pretty sure that this method can be adapted for RNA (use the lysis conditions then do a trizol purification instead of the phenol choloroform): http://jvdi.org/cgi/reprint/13/2/162.pdf

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by canalon
Sat Jul 25, 2009 1:28 am
 
Forum: Molecular Biology
Topic: RNA from feathers
Replies: 3
Views: 140

Re: How to be "good at the bench"

... biological purposes. Sterilization 1. Autoclave most buffers, and undefined bacterial and yeast media 2. Don’t autoclave: corrosives (Acids, phenol, bases), solvents or volatiles (ethanol, methanol. Chloroform); liquids containing bleach, formalin, or glutaraldehyde; buffers with detergents, ...

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by jangajarn
Sat May 23, 2009 7:15 pm
 
Forum: General Discussion
Topic: How to be "good at the bench"
Replies: 3
Views: 1440

PHENOL-CHLOROFORM EXTRACTION USING UNPASTEURISED STILTON!

... in cheese or dairy, I am pretty sure that you might find some help. A quick search in Pubmed suggest that some steps are added to the regular phenol/chloroform protocol.

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by canalon
Sun Apr 26, 2009 7:43 pm
 
Forum: Molecular Biology
Topic: PHENOL-CHLOROFORM EXTRACTION USING UNPASTEURISED STILTON!
Replies: 1
Views: 377

Identifying unknown

... oxygen and with oxygen. It grew on MacConkey agar but not on mannitol salts agar. No amylase. It is a fermenter. It produced acid but no gas in a phenol-red with glucose containing a Durham tube. MR+/VP-. In a triple sugar iron test the butt was yellow, slant pink and produce minimal gas. Indole ...

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by mccullyj
Fri Apr 24, 2009 10:22 pm
 
Forum: Microbiology
Topic: Identifying unknown
Replies: 1
Views: 1135

PHENOL-CHLOROFORM EXTRACTION USING UNPASTEURISED STILTON!

... and characterisation of non-starter lactic acid bacteria in unpasteurised Stilton cheese'. After carrying out various attempts using a standard phenol-chloroform DNA extraction....it did not work! I was wondering if anybody coulod give me any idea's on why it didnt work? All reagents were new ...

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by UGmicro
Fri Apr 24, 2009 5:05 pm
 
Forum: Molecular Biology
Topic: PHENOL-CHLOROFORM EXTRACTION USING UNPASTEURISED STILTON!
Replies: 1
Views: 377
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