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Dictionary » P » Pas stain Pas stainPAS stain --> periodic acid Schiff stain (Science: technique) A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping. It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product. Substances that can be demonstrated include carbohydrates, mucins, cartilage matrix, collagen, reticulum, basement membranes, fibrin, thyroid colloid, amyloid, glomerular hyaline deposits, and a number of other secretions or tissue constituents. Also used in for staining gels on which glycoproteins have been run. See: periodic acid Schiff reaction Synonym: pAS stain. ![]()
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Results from our forumseparation problem with GelRedI am a bit surprised here, you say that post satining is too time consuming but you are ready to slow down your gels. It takes 10 minutes for staining, and about as much if you want to destain, which should not even be a problem with gelred. So why not post stain? If this is really not an option, ...
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Re: Methods Identifying Unknown Bacteria?Gram's stain is certainly useful in classification but not always truthful. Some organisms, Acinetobacter in particular, can readily stain gram-positive in fresh clinical material, yet stains gram-negative after growth on plated ...
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Methods Identifying Unknown Bacteria?Isn't the first thing to do is a Gram stain? And then find out if it is anaerobic, aerobic, or facultative? then look at it under a microscope for its shape? what about the media that it can grow on? put different antibiotics in the media and ...
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i need this article please help mewell i'm doing an work with imageJ software with colour deconvolution (detection of DAB stain) and i need for validity this article: Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol 23: 291-299, 2001 if someone has this article ...
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Some problems of DNA recovery from native PAGE gel... recovery is as follows, 1. 20% native PAGE gel (The DNA i want to purify is around 20bp dsDNA + 10 bp ssDNA ) 2. running at 250 v for 30mins, 3. stain and cut the desired band; 4. cursh the gel by yellow tips into small gains; 5. add 2volume TE buffer (100ug gel ~ 200ul TE buffer)., rotating ...
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