Paraffin

Hi, eveyone, I tried to do CD34 membrane antigen retrieval from paraffin embedded tissue section with microwave and enzyme methods, but just found these methods are not working. The protocols I used are as follows. http://www.e-biotek.com/immunology/241-immunohistochemistry-retrievals.html ...

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... NIMP-R14 The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. - Sa14-2 Sa14-2 is a monoclonal antibody that reacts with the mouse monocyte marker CD14.

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Ive been working in a PATH lab for 2 months now. I have been given the assignment of extracting RNA from Formalin fixed Paraffin tissue. So far I have used the RNAeasy Kit for RNA extraction from FFPE and i have also tried a TRIZOL method. NOTHING IS WORKING!!!! I get 0 bands after ...

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Have you tried Formapure from Agencourt Biosciences? This kit is designed for RNA extraction from paraffin embedded tissue for real time PCR or microarray use. Has anyone else tried extracting RNA from Formilin fixed parafin tissue? and do you know of an optimal protocol ...

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... both of which are supposed to be quite abundant on the surface of regular T cells in the lymph node (this is a control). First, I tried to stain paraffin sections using a protocol given to me by another lab member who's done some staining before (the protocol is not very specific). I stained ...

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