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Oxidase

oxidase

Classically, one of a group of enzymes, now termed oxidoreductases (EC class 1), that bring about oxidation by the addition of oxygen to a metabolite or by the removal of hydrogen or of one or more electrons. Oxidase is now used for those cases in which O2 acts as an acceptor (of H or of electrons); those removing hydrogen are now termed dehydrogenases. For individual oxidases, see the specific names.

direct oxidase, originally, an oxidase catalyzing the transfer of O2 directly to other bodies; now termed oxygenase.

indirect oxidase, originally, an oxidase that acts by reducing a peroxide; now termed peroxidase.

terminal oxidase, the last protein in the electron transport, respiratory chain. In mammals this is cytochrome c oxidase. Any of the enzymes that catalyze biological oxidation.Oxidase is an [[enzyme which is capable of adding [[ oxygen to a substance, where hydrogen is usually released as a by-product which can bind with oxygen to form water.


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Test on unknown bacteria

Hi I would like to find out is it possible if i do a Cytochrome oxidase c test on gram positive bacteria then use the entero tube for those test negtive and oxi/ferm tube for the posiive ones? bcz i've been finding only this test can be done on gram negative ...

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by damm182
Tue Aug 04, 2009 4:34 am
 
Forum: Microbiology
Topic: Test on unknown bacteria
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Views: 120

help identify unknow species

... help,please. Here is what I have done in lab and here are my results: Gram (-) rod strict aerobic MacConey : Gram-,Lac- motile (+) endospore(-) oxidase(+) catalase(-) H2S(-) reduction of nitrate and nitrite(-) glucose fermentation (-) lactose (-) sucrose(-) mannitol(-) MR(-) VP(-) starch hydrolysis ...

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by 198839
Sat Jul 11, 2009 12:26 am
 
Forum: Microbiology
Topic: help identify unknow species
Replies: 2
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Re: Unknown Bacteris Identification

i believe the oxidase test is truly negative and not positive so i'm leaning towards proteus vulgaris. let me know if anyone thinks different. Thanks.

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by cmuguerrero
Mon May 11, 2009 3:01 am
 
Forum: Microbiology
Topic: Unknown Bacteris Identification
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Views: 766

Unknown Bacteris Identification

... (+) lactose (-) starch hydrolysis (+) casein hydrolysis (+) indole (+) methyl red (+) V-P (-) citrate (+) nitrate (+) urease (+) catalase (+) oxidase (+) Any help is greatly appreciated?

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by cmuguerrero
Mon May 11, 2009 12:45 am
 
Forum: Microbiology
Topic: Unknown Bacteris Identification
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Identifying unknown

... tube. MR+/VP-. In a triple sugar iron test the butt was yellow, slant pink and produce minimal gas. Indole test positive. It reduced nitrate. Oxidase negative, catalase positive but weak, coagulase negative, b hemolitic and had motility. Motility test done in motility medium. If anyone has ...

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by mccullyj
Fri Apr 24, 2009 10:22 pm
 
Forum: Microbiology
Topic: Identifying unknown
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