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Dictionary » N » Nickel Nickelnickel 1. (Science: chemistry) A bright silver-white metallic element. It is of the iron group, and is hard, malleable, and ductile. It occurs combined with sulphur in millerite, with arsenic in the mineral niccolite, and with arsenic and sulphur in nickel glance. Symbol ni. Atomic weight 58.6. On account of its permanence in air and inertness to oxidation, it is used in the smaller coins, for plating iron, brass, etc, for chemical apparatus, and in certain alloys, as german silver. It is magnetic, and is very frequently accompanied by cobalt, both being found in meteoric iron. 2. A small coin made of or containing nickel; especially, a five-cent piece. Nickel silver, an alloy of nickel, copper, and zinc; usually called german silver. Synonym: argentan. Origin: G, fr. Sw. Nickel, abbrev. From Sw. Kopparnickel copper-nickel, a name given in derision, as it was thought to be a base ore of copper. The origin of the second part of the word is uncertain. Cf. Kupfer-nickel, Copper-nickel. ![]()
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Results from our forumG genes... understand the following things: - Protein G genes - Recombinant His-tagged proteins (and how they are purified by affinity chromatography on a nickel-agarose column) Thank you very much for your help! - Tim
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Re:... now correlate that which iron antagonists and synergists, http://www.acu-cell.com/femn.html Iron Synergists: Phosphorus, bismuth, germanium,nickel, manganese, Vitamin A, Vitamin B1, Vitamin C, folate, niacin, niacinamide, lecithin, protein, Iron Antagonists: Zinc, calcium, magnesium, tin, ...
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experiences with ni-affinity protein purification??Hi! I´m trying to purify a his-tagged protein with nickel-columns. When I use imidazol in the elution buffer my enzyme is inactive after purification. I showed that imidazol is the reason for this loss of function. Because before purification I can ...
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Re: inactive enzyme after purification with Ni-TEDThere are a number of potential problems with nickel chelating resins and metalloproteins. First, the background buffer is typically phosphate which can form soluble and insoluble complexes with metals, leaching them off the protein in the process. ...
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6X His Tag vector construction assistance neededI think it depends. If you are using Qiagen's NI-NTA system (or some other nickel or cobalt-based system) to purify the His-tagged protein, it shouldn't matter for that step whether there are a few extra aas at the N terminus before the 6xHis tag. However, you ...
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