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Mutagenic

mutagenic

(Science: genetics molecular biology) Inducing genetic mutation.

Origin: Gr. Gennan = to produce


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Uses for the PCR technique?

... of the technique: For plasmid manipulations, this technique has largely been supplanted by a PCR-like technique where a pair of complementary mutagenic primers is used to amplify the entire plasmid. This generates a nicked, circular DNA which can undergo repair by endogenous bacterial machinery. ...

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by MrMistery
Mon May 04, 2009 11:41 pm
 
Forum: Genetics
Topic: Uses for the PCR technique?
Replies: 4
Views: 1343

New to research and confused! Help? :-)

... that contains a mutation in the lacZ gene that produces "white" colonies on X-gal/IPTG plates. The manufacturer supplies you with two mutagenic oligonucleotides that, if everything works, will convert a cryptic stop codon to the correct amino acid and produce blue colonies on X-gal/IPTG ...

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by blcr11
Fri Jun 08, 2007 10:27 pm
 
Forum: Molecular Biology
Topic: New to research and confused! Help? :-)
Replies: 5
Views: 960

New to research and confused! Help? :-)

... what you’re doing—is to use something like a QuikChange kit from Stratagene (soon to be part of Agilent). The process involves pcr with at least 2 mutagenic primers, but you shouldn’t have to digest out any fragments unless you’re doing some kind of cassette-style transfers to other vectors. It ...

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by blcr11
Fri Jun 08, 2007 8:32 am
 
Forum: Molecular Biology
Topic: New to research and confused! Help? :-)
Replies: 5
Views: 960

The Fiber Disease

... to all man-made environmental electromagnetic pollution. Reference 2 proves that the magnetic component of electromagnetic pollution can be mutagenic in human tissue. This is the great danger of electromagnetic pollution. We then describe the three components of the electromagnetic pollution ...

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by Nadas Moksha
Tue Dec 12, 2006 5:23 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748648

The Fiber Disease

... , intelligent design is hard to swallo... or a hybred explanation of the global contamination of ectoparasitic picoscopic silion life haboring mutagenic radiological self synthesising photonic fibril self organising into super macrophage by complex circutry and Q sensing forming an atom thin ...

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by Nadas Moksha
Mon Dec 11, 2006 6:00 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748648
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