Metabolise

... if it is a CYP inducer, but... can an inhibitor have such a profound effect that the cell attempts to compensate for the loss in the ability to metabolise substances that it upregulates the synthesis of the CYP? I've been googling for the past few hours, but nothing solid has come up :(

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Viruses can replicate, and some viruses can metabolize, so they are lives. Viruses do not metabolise. They reproduce and this only when inside the host cell. The host cell synthesises the proteins for the virus using the cell's ribosomes. Are viruses alive? If it were ...

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... yeast, but more. If that is the case, then honestly you're fucked. Unless you can find something that your target yeast can do that others cannot(metabolise something, grow in the presence of something, grow in the absence of something etc). Your best bet would be to hope that you get exctly what ...

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... desired cells. Again, this can be something that can be readily visualised (like GFP) or only permits transfected cells to survive (an ability to metabolise certain nutrients or toxins that the wild type cells cannot, for example). And of course you need to verify that the cells produce whatever ...

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... want to keept them in their ideal temperature (~ +37C for human cells, twenty-something for insect cells etc.), because that allows the cells to metabolise and divide. There are other reasons for keeping PBS cold as well, e.g. fluorescent antibody labels retain their intensity and binding (and ...

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