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Lysozyme

Lysozyme

(Science: cell biology, enzyme) Glycosidase that hydrolyses the bond between N acetyl muramic acid and N acetyl glucosamine, thus cleaving an important polymer of the cell wall of many bacteria. Present in tears, saliva and in the lysomes of phagocytic cells, it is an important antibacterial defence, particularly against gram-positive bacteria. An enzyme found in saliva and sweat and tears that destroys the cell walls of certain bacteria.An enzyme capable of breaking down cell walls that is used in the immune system to eliminate foreign microbes with its powerful digestive properties.


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Lost Protein?

... on my current situation. I have confirmed the recombinant protein I'm working to collect is expressed in T7 ecoli/pET24a. And after addition of lysozyme and then sonication the supernatant also showed the expected protein. INFO: the protein's iso electric point is predicted to be 9.8... the ...

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by BrianScott
Tue Jun 30, 2009 4:05 pm
 
Forum: Molecular Biology
Topic: Lost Protein?
Replies: 0
Views: 143

Cell wall lysis

Lysozyme, or concentrated lysozyme, at least, may help. Oddly enough, it is in your saliva, and it lysis cell walls. I don't actually know if it would work, so maybe someone will criticize me and provide a better answer.... ...

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by plasmodesmata11
Thu Apr 09, 2009 9:30 pm
 
Forum: Cell Biology
Topic: Cell wall lysis
Replies: 3
Views: 528

How can I determine lysozyme activity?

Hello,Did somebody use EnzChek Lysozyme Assay Kit(invitrogen,molecular probes)? I meet some difficulty in determining lysozyme activity using the EnzChek Lysozyme Assay Kit(invitrogen,molecular probes). the fluorescence of samples is very ...

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by angelsmile
Tue Oct 21, 2008 2:30 am
 
Forum: Microbiology
Topic: How can I determine lysozyme activity?
Replies: 0
Views: 880

lysozyme doubt...

agar LYSOZYME csf me hota to kya hota?

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by sonawanes61
Fri Mar 14, 2008 9:59 am
 
Forum: Molecular Biology
Topic: lysozyme doubt...
Replies: 2
Views: 833

SDS

I understand that it denatures proteins. but bacteria were lysed by lysozyme and lysis buffer, why need sds?

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by okmedcui
Sun Jan 27, 2008 4:20 pm
 
Forum: Genetics
Topic: SDS
Replies: 4
Views: 755
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