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Lysis

Definition

noun

(cytology)

The disintegration or rupture of the cell membrane, resulting in the release of cell contents or the subsequent death of the cell.

(medicine)

The gradual recovery or recuperation from an acute disease.

(suffix: -lysis)

Decomposition, breakdown or disintegration.


Supplement

(cytology) Lysis occurs as a result of viral or osmotic mechanisms that compromise the integrity of the cell membrane.


Word origin: from Greek λύσις, lysis from lyein = to separate. Related forms: lyse (verb), lytic (adjective).
Derived terms: cytolysis, dermatolysis, haemodialysis, haemolysis, enzymolysis, hydrolysis.


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RNA from feathers

... from feather in Google. Like that: http://jvdi.org/cgi/reprint/13/2/162.pdf and I am pretty sure that this method can be adapted for RNA (use the lysis conditions then do a trizol purification instead of the phenol choloroform): http://jvdi.org/cgi/reprint/13/2/162.pdf

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by canalon
Sat Jul 25, 2009 1:28 am
 
Forum: Molecular Biology
Topic: RNA from feathers
Replies: 3
Views: 138

Problems related to Microsome Preparation

... as I am troulbed by the absence of results!!! I used LCC6MDR to take microsomes from fragement of plasma membrane. I lysed the cells by lysis buffer first and then centrifuged it at 500g for 10 min in cold room to take supernatant use another centrifugation at 5000g for 10 min in cold ...

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by benzenefancy
Tue Jun 30, 2009 7:21 pm
 
Forum: Molecular Biology
Topic: Problems related to Microsome Preparation
Replies: 0
Views: 140

Separation of microsome by centrifugation

... as I am troulbed by the absence of results!!! I used LCC6MDR to take microsomes from fragement of plasma membrane. I lysed the cells by lysis buffer first and then centrifuged it at 500g for 10 min in cold room to take supernatant use another centrifugation at 5000g for 10 min in cold ...

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by benzenefancy
Tue Jun 30, 2009 7:06 pm
 
Forum: General Discussion
Topic: Separation of microsome by centrifugation
Replies: 0
Views: 65

Question about Apoptosis

well, I know that apoptosis is programmed cell death, whereas lysis is the breaking or destruction of a cell

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by plasmodesmata11
Sun Apr 19, 2009 11:12 pm
 
Forum: Cell Biology
Topic: Question about Apoptosis
Replies: 1
Views: 221

Question about Apoptosis

... confused. Do perforins kill the cell, or does it send a signal and tell the cell to kill itself? And what is the difference between apoptosis and lysis? I'm a first year biology student, so if you can explain this is simple terms, that'd be great!

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by lisczz
Sun Apr 19, 2009 5:56 pm
 
Forum: Cell Biology
Topic: Question about Apoptosis
Replies: 1
Views: 221
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