Ligases

After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase... do you need to select a specific DNA ligase that matches with the specific restriction enzyme that was used?

See entire post

... and an enzyme Commission (EC) number. They are divided into six main groups, oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. Any of several complex proteins that are produced by cells and act as catalysts in specific biochemical reactions.A type of protein that catalyses ...

See entire post

... called plasmid. In the plasmid you can insert DNA fragments by opening it by restriction enzymes and then closing plasmid with fragment by ligases; and let them grow to create multiple copies; or alternately they can be easly cultivated and lysed to extract their DNA with insert; DNA extracted ...

See entire post

i thought recombinant DNA was attached 2gether by the same restriction endonuclease used 2 break it. i never knew DNA ligases took part in anything but in the replication and repair of DNA. some1 correct me if i'm wrong plz.

See entire post

Hmms yea agreed.. DNa ligases should be up to the task - but it all depends on whether the cut strands are blunt ends or sticky ends :D

See entire post