Lambda

... an experiment to determine the rate at which the TdT added dNTPs to the 3’ end of DNA, the following experiment was set up: Linear-bacteriophage lambda DNA (48.5kb) at a concentration of 55ug ml^-1 was cut with the restriction enzyme Alu I. (Lambda DNA is cut 143 times with Alu I). The following ...

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... using a library approach? I understand what a library is, but what would you do with a library to identify splice variants? I know you can use lambda zap kit from Stratagene, but I'm not clear on what it does! Can anyone help me out with the basic concept here? Thanks!

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... you that much, Cat. I’d still bet on rRNA, though on further reflection, the question may not be answerable with the information given. I see that lambda exonuclease cannot initiate digestion from a nick or a gap (although it does have very weak activity against ssDNA or so says the descriptive ...

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This question is unfair. 1) resistant to digestion with lambda exonuclease: lambda exonuclease has 5' to 3' activity on double stranded DNA so all choices given would be resistant since 3 of them are circular DNA and 1 is RNA; 2) it does not show typical ...

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... for “replicative” form, though I’m not quite sure what the II is for) is a ds circle, although the infective DNA is ss and would be resistant to lambda exonuclease. rRNA should be resistant to the exonuclease, does not have a typical melting curve, and is very dense compared to typical duplex ...

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