Introns

genomic DNA..actually genes have exons+introns...cDNA you get from mRNA(thus it contains only exons).If you mix genomic and cDNA and heat them..they will denaturate..then you lower temperature and cDNA will hybridize with genomic DNA on matching ...

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As we know all introns obey the GT-AG rule (donor and acceptor splice site). I would like to know what actually happen if the GT-AG sites were intervened with unusual junction sequences (before GT/after AG), for eg: 1. gcgcgggc T ...

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... organisms. The best nuclear DNA to use for determining relationships between closely related indivduals is, again, the most variable regions. Introns, pseudogenes, MHC genes would be your best bets. May I ask what species you're working with? And what your goal is? Microsatellites might be ...

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The coding strand of the DNA is made up of introns (non-coding parts) and exons (coding parts). when the mRNA is transcripted from the DNA it includes all the corresponding parts (both the introns and exons) but then the mRNA is spliced, which ...

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hi canalon, thanks for your hints. i think i know my answer now. ub2 is not a separate gene but the exon that wasn't included in the splicing process. it was annotated as a gene, but may not necessarily a gene. although there is no cDNA in the database encoding for the gene, the 123 amino acids are ...

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