In situ

Hi guys, I'm preparing the probe for in situ hybridization. It's the product from degenerated PCR so i guess it's ssDNA. I labeled the probe with DIG, keep in 50%formamide and hybridization buffer with carrier DNA. I was so stupid that I left ...

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... SSCP PCR RFLP Variants of PCR (1) AFLP PCR Alu-PCR Asymmetric PCR Colony PCR Protocols Competitive and/or Quantitative RT-PCR Degenerate PCR In Situ PCR Differential Display PCR Inverse PCR Ligation Mediated Suppression PCR Long PCR Protocols Methylation Specific PCR Multiplex PCR Nested PCR ...

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... interstitial cells. Leydig cells, which comprise up to 80% of the testicular intertubular cell population, are known to synthesize testosterone in situ. Indirect evidence indicated that an oxytocin-like peptide was also present in Leydig cells. The question arose whether this peptide was synthesized ...

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Brace yourselves for the question I was too embarassed to ask my labmates. The lab protocol for producing in situ hybridization probes calls for precipitation in lithium chloride solution in order to "clean up" the RNA after DIG-labeling transcription. The protocol ...

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I am in the process of making RNA probes (900bp) for in situ. I have cloned my gene (DUSP 5) which 900 bp into a pBsk vector. I have sequenced the gene using M13 F/R, T3/ T7 and my own designed primers. When I sequence from the '3 end I get perfect match ...

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