Impurities

Pig insulin is almost human insulin with difference of only one amino acid.The cause for immune response isn't the insulin itself,but impurities in the insulin.Even "DNA-recombinent" synthesized insulin_which is referred as human insulin_can cause allergy because of impurities of ...

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You need to purify your DNA prior to running your gel. Salts and other impurities can interfere with the electrophoresis. :shock: Never heard that. I use electrophoresis to purify DNA... As for the original question. Besides contamination of reagents by DNAse ...

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You need to purify your DNA prior to running your gel. Salts and other impurities can interfere with the electrophoresis.

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The main purpose of running the fragment on the gel is to remove impurities, like you said: you can cut out the band (assuming there are multiple bands) that contains the fragment you want and extract it from the gel. You should always run a gel of your ...

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... ligation and transformation. Why A-addition is not performed directly on the amplicon? Is it mainly to get pure DNA because there might be impurities in the PCR reaction?! Or is it a double check for the size of amplicon? Thanks for your help.

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