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Dictionary » H » Hybridisation

Hybridisation

Definition

noun

(biology) The act or process of mating organisms of different varieties or species to create a hybrid.

(molecular biology) The process of forming a double-stranded nucleic acid from two complementary strands of DNA (or RNA).

(chemistry) The mixing of atomic orbitals to form new orbitals suitable for bonding.


Supplement

In biology, hybridization (or hybridisation) is usually used as a term in agriculture or in plant production where new hardy and disease-resistant crops are formed.


Variant: hybridization.
Related terms: hybrid (noun), hybridize or hybridise (verb).


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DNA hybridisation

Can anyone help me here Iam struggling to understand how to calculate the amount of DNA probe i need for my hybridization solution in a blot. say for a hybridization solution of 450 microliter, I have 0.5pmol DNA probe, what volume of the probe i need for this hybridization solution?

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by bizy
Tue Jun 30, 2009 6:00 am
 
Forum: Molecular Biology
Topic: DNA hybridisation
Replies: 0
Views: 123

clearing up terminology

I have a paper to write on the molecular anaylsis advancements of histopathology such as in situ hybridisation and in situ PCR. however when i look these methods up, they fall under cytogenetics rather than histopathology. I wanted to know if cytogentics is part of histopathology ...

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by aftereleven
Fri Jan 04, 2008 5:15 pm
 
Forum: Molecular Biology
Topic: clearing up terminology
Replies: 1
Views: 663

short dsDNA purification

Hi, Thank you so much. I'll have a look to the papers. Concerning the ssDNA hybridisation, we are already trying but it seems to not work properly. Have you any reference about it? Cheers, Davide

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by dldavide
Thu Jul 19, 2007 9:01 am
 
Forum: Molecular Biology
Topic: short dsDNA purification
Replies: 5
Views: 744

OMG PLEASE HELP.. QUESTIONS FOR FINAL...

Thanks... k next Why hypotheses did Gregor Mendel develop based on his observations of pea plants? beacause all the results of selfing and hybridisation experiments were relative to each other thats why.....

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by sachin
Sat Dec 16, 2006 7:03 pm
 
Forum: Cell Biology
Topic: OMG PLEASE HELP.. QUESTIONS FOR FINAL...
Replies: 26
Views: 1651

The Fiber Disease

... from the parasitoid wasp Pimpla hypochondriaca has potent in vivo activity against insect haemocytes and disrupts host immune responses. Using hybridisation techniques, and more recently random sequence analysis, we had previously identified cDNAs encoding 10 venom proteins from this wasp and ...

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by London
Sun Oct 01, 2006 11:18 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748693
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