Holoenzyme

... activation of different regulons, and this is used in the many stress responses (e.g. of e.coli) But, on the genome, can operons induced by the holoenzyme incorporating one sigma factor overlap those that are activated as the result of another sigma factor? If not, why not, and if yes, then ...

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... the end of one protein coding region and another, then what is responsible for the termination of the transcription process - i.e. how would the holoenzyme know when to stop transcribing the DNA? Thanks very much in advance! Neon

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... in an operon (operonA), then this requires (amongst other things) RNAP and a sigma factor - call this sfA. When sfA and RNAP bind and form the holoenzyme, the genes in operonA are transcribed. (ignore any activators/repressors for now). OperonB contains promB etc...If only sfA is present, the ...

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... ori, the leading strand will be leading throught its replication and the lagging will be lagging. The replication enzyme being employed is not the holoenzyme E.Coli PolI but its Klenow fragment. A Klenow fragment lacks 'nick-translation' activity. Therefore the RNA primers are not degraded. Hence ...

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... formed (without the helper molecule) is called apoenzyme. It is not active. when the coenzyme or cofactor binds to the structure it is called holoenzyme. Holoenzyme is the active enzyme. Hope you get the aswer. :)

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