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Hairpin

Hairpin

The structure formed by a polynucleic acid by base-pairing between neighboring complementary sequences of a single strand of either dna or rna.


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Crystallization of small RNA Hairpin Structures

... down and annealing they bond with neighboring RNA sequences instead of the sequence from their own chain, preventing the presentation of the hairpin in any crystals that form. Am I overlooking anything from the crystallization process of RNA, or does anyone else have any ideas? ex. of GUAA ...

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by cgh24
Mon Mar 09, 2009 6:56 pm
 
Forum: Cell Biology
Topic: Crystallization of small RNA Hairpin Structures
Replies: 0
Views: 269

Re: rna interference

... the vial RNA-directed RNA polymerases. However, when RNAi is produced from animal genomes, the double-stranded RNA is manufactured by cleaving a hairpin twice. In animals, the RNA is transcribed as a single strand of RNA which contains a self-complementary region, and after the self-complementary ...

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by jonmoulton
Mon Feb 09, 2009 5:17 pm
 
Forum: Molecular Biology
Topic: rna interference
Replies: 4
Views: 722

Partially double-stranded RNA molecule question......

Hairpin loop structure are quite common. In fact tRNA are quite interesting in this respect. As for what meakes this possible, I would say Watson-Crick pairs. RNA is just like DNA in this respect.

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by canalon
Mon Oct 08, 2007 8:03 pm
 
Forum: Genetics
Topic: Partially double-stranded RNA molecule question......
Replies: 1
Views: 644

short dsDNA purification

... ends prepared by T4 DNA polymerase's error-correcting activity. I suspect the problem with using long stretches of ssDNA is the potential for hairpin formation, especially near the ends, that might interfere with hybridization. I would use sites with as long an overhang as I can get and then ...

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by blcr11
Thu Jul 19, 2007 11:48 am
 
Forum: Molecular Biology
Topic: short dsDNA purification
Replies: 5
Views: 744

how to detect dsRNA ?

... double-stranded, whereas miRNAs are single-stranded. Although initially single stranded there are regions of intra-molecular association causing hairpin structures in pre-miRNAs; immature miRNAs. Very recently, dsRNA has been found to induce gene expression at transcriptional level, a phenomenon ...

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by dipjyoti
Sun Feb 18, 2007 10:47 am
 
Forum: Molecular Biology
Topic: how to detect dsRNA ?
Replies: 6
Views: 955
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