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Glutamine

A crystalline amino acid occurring in proteins; important in protein metabolism.One of the 20 amino acids that are commonly found in proteins.glutamine

(Science: amino acids) One of the 20 amino acids commonly found (and directly coded for) in proteins. It is the amide at the _ carboxyl of the amino acid glutamate. Glutamine can participate in covalent cross linking reactions between proteins, by forming peptide like bonds by a transamidation reaction with lysine residues. This reaction, catalysed by clotting factor XIII stabilises the aggregates of fibrin formed during blood clotting. Media for culture of animal cells contain some 10 times more glutamine than other amino acids, the excess presumably acting as a carbon source.


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[molecular bio]calculation

1 - use the well known formula: C1*V1=C2*V2 you know C2 (5mM), V2 (4ml) and V1 (50ul) solve for C2 2 - Calculate how many (milli)moles of Glutamine are needed to adjust the concentration in 1L (1mM=1mol.L) correct for the volume (express in liter...) and then you can easily figure out how much ...

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by canalon
Sat Aug 15, 2009 1:16 pm
 
Forum: Molecular Biology
Topic: [molecular bio]calculation
Replies: 3
Views: 122

[molecular bio]calculation

... would you make up the stock solution? 2.A particular cell line will grow well in a standard medium (DMEM) provided it is supplemented with extra glutamine. The standard medium contains 1 mM glutamine but this cell line grows better with 5 mM glutamine. How much more glutamine would you need to ...

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by scholesmu
Sat Aug 15, 2009 9:48 am
 
Forum: Molecular Biology
Topic: [molecular bio]calculation
Replies: 3
Views: 122

Re:

... Thanks mith, one more thing though, is there any way of stopping the process before the ammonia is bonded to glutamate and becomes glutamine thus getting pure ammonia?

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by octavian
Fri Aug 01, 2008 12:08 am
 
Forum: Microbiology
Topic: Ammonia producing bacteria/microbes
Replies: 3
Views: 1548

cell separation

the composition of medium is for 50 ml: 43,5 ml RPMI + 5 ml FBS + O,5 ml MEM + o,5 ml L-GLUTAMINE + O,5 ml PENICILLIN-STREPTOMYCIN. The number of cells was over-estimated by a factor of 5. After the counting,we divide the number of cells by 20 million for knowing the ...

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by tregis6
Tue Jun 24, 2008 12:03 pm
 
Forum: Cell Biology
Topic: cell separation
Replies: 3
Views: 923

Sugar Allergy

... the gastric mucosal toxicity of aspirin in human subjects. Am Coll Gastroenterol 1999;94(7):1818-22. 28. Ziegler TR, Bazargan N, Leader LM, et al. Glutamine and the gastrointestinal tract. Curr Opin Clin Nutr Metab Care 2000;3(5):355-62. 29. Klimberg VS, Salloum RM, Kasper M, et al. Oral glutamine ...

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by DrDahlman
Sat Jan 12, 2008 4:20 pm
 
Forum: Human Biology
Topic: Sugar Allergy
Replies: 71
Views: 68328
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