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Globin

Globin

(Science: protein) The polypeptide moiety of haemoglobin. In the adult human the haemoglobin molecule has two _ (141 residues) and two _ (146 residues) globin chains.


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Lentivirus vector propagation

Hi I bought a lentivirus vector containing alpha globin gene and I want to grow this vector. In what bug I should propagate it?

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by roniadam
Mon Jun 29, 2009 7:11 am
 
Forum: Molecular Biology
Topic: Lentivirus vector propagation
Replies: 4
Views: 182

Re: Any SOLID arguments against evolution?

Futurezoologist in regards to hemoglobin A vast number of ways yes, but i think that you think it is much more than it is, you haven't taken into account polar bonds(of which there are a lot in hemoglobin due to H, N, O), the stress put on ...

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by AFJ
Tue Jun 02, 2009 1:19 am
 
Forum: Evolution
Topic: Any SOLID arguments against evolution?
Replies: 106
Views: 16676

Gene expression

... we know every gene has an allele on each chromosome. My question is that if we have a protein that is produced by more than one gene (e.g. alpha globin wich is produced by alpha 1 and alpha 2 genes on each chromosome). In this case we will have 4 genes from both chromosome. How the globin will ...

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by roniadam
Sat Mar 28, 2009 2:35 am
 
Forum: Molecular Biology
Topic: Gene expression
Replies: 1
Views: 418

Re: Proving Existence of Introns!

Here is a very fun way. Black is your cDNA, red is radioactively labeled chromosomal DNA containing the gene for Beta globin. You first hybridize them. then treat with either S1 nuclease or exonuclease VII. S1 nuclease is an enzyme that degrades all single stranded DNA, exonuclease ...

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by MrMistery
Thu Jan 22, 2009 2:48 am
 
Forum: Genetics
Topic: Proving Existence of Introns!
Replies: 4
Views: 479

Proving Existence of Introns!

Using today's technology, how would a person prove the existence of introns starting with the cDNA of B-globin? I was thinking about using a microarray approach. Have oligos from the chromosomal gene on the slide and hybridize the cDNA to it. If introns do exist, then there ...

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by BioGradStudent
Sun Jan 11, 2009 11:47 pm
 
Forum: Genetics
Topic: Proving Existence of Introns!
Replies: 4
Views: 479
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