Fluorescent

... DNA/mL. With special assays you can go down to 5 ng/mL. Other options would be Ethidium bromide (which is around 10 times less sensitive) or the fluorescent dye SybrGreen.

See entire post

... cells are darker which grew up with (A) then it would show that (A) does increase cell permeability. Another idea would be to somehow attach a fluorescent tag or even light emitting tag to the the chemical (B) and then measure either the amount of light given off or just view and see if the ...

See entire post

... Here I would like to ask something about the FSC-A oscillograph trace. About my research, I use the BT-20 cell line, and after incubation with my fluorescent proble, I collect the cells and apply them to the FACS Canto apparatus. But no matter how I adjusted the parameters, I got 2 peaks in the ...

See entire post

... not exceed 250 bp. But i dont see why that should be a problem since a normal PCR can have much larger amplicons. Has it anything to do with the fluorescent dye?

See entire post

... brings us back to the original question. (1) - In DNA sequencing, how come scientists just don't tag regular deoxyribonucleotides with a specific fluorescent molecule (so that each type of nucleotide get a different color - one for A, one for G, one for T, and one for C) so that the DNA polymerase ...

See entire post