Fixative

PFA is just a fixative. Beware that PFA may cause autofluorescence and give you a lot of unwanted background staining. Triton X-100 is good for permeabilization. Adding some Tween 20 will help as well.

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Routine histological method: paraffin method with fixative formaldehyde and standars PAS staining for beta cell.

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... is successful, we still need to process for preparation. This means we will face another problem because organs/tissues sometime get shrinked in fixative or another chemicals in the process. I cannot imagine if that tiny baby will be shrinked. I will get nothing to examine by then :(

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... 40%...:lol: What about the location? If the arena is at the shore, cepha can "run" when their chemical cencor cells touch your fixative. If the arena is in terrestrial area, cheli will win :P

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... (placebos). On day 35 and 68, mice were sacrificed. Testes were taken and were processed for histological preparation in paraffin method using fixative Bouin and HE staining. Number of spermatogonia, spermatocytes, spermatids, and total spermatogenic cells were counted. Data were analyzed statistically ...

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