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Fdnb

Fdnb --> fluoro-2,4-dinitrobenzene

a reagent used to combine with the free NH2 group of the NH2-terminal amino acid residue in a peptide, thus marking this residue; the combined forms are known as dnp-proteins, dnp-aminoacyl, etc., the fluorine having been replaced to leave a dinitrophenyl residue (dnp, dnp, or N2Ph-) attached to the NH2 group.

Synonym: Sanger's reagent.

Acronym: fdnb


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Protein sequence determination

Maybe it's not quite fair to say you can't sequence with FDNB. That's true as far as it goes, but with some additional chemistry and ingenuity you can sequence peptides, but you need something more than just FDNB. You need to be able to isolate peptides. ...

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by blcr11
Thu Sep 18, 2008 1:20 pm
 
Forum: Molecular Biology
Topic: Protein sequence determination
Replies: 2
Views: 936

Protein sequence determination

You can't really sequence a protein with FDNB. Sanger's Reagent (FDNB) was useful in the "old" days of protein work because it gives a clean reaction with free primary and secondary amine groups. DNP-amino acids are stable under acid ...

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by blcr11
Tue Sep 16, 2008 5:33 pm
 
Forum: Molecular Biology
Topic: Protein sequence determination
Replies: 2
Views: 936

Protein sequence determination

How is FDNB used to determine the sequence of a polypeptide? (which is the reaction or what happens to the aminoacids)

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by Ricarda
Fri Sep 12, 2008 8:03 pm
 
Forum: Molecular Biology
Topic: Protein sequence determination
Replies: 2
Views: 936


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