Extraction

... suspect that you can use pretty much any organic solvent with good results. Here is a link http://www.monsanto.com/pdf/responsibility/chlorophyll_extraction.pdf to a protocol describing chlorophyll extraction using isopropanol. Cheers, Andrei

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... expect more bands not too less if you had a contamination. In my opinion the only thinkable explanation involving a contamination would be if the extraction of the cellular DNA went wrong. Do you have the gene already in a plasmid anywhere in your lab? We once found out that we had plasmid contamination ...

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I have ordered samples of plant DNA extraction kits from both MoBio and Epicenter. I am looking forward to try those. I noticed that on the gel I ran for my genomic extract that there was much more intensity of bands that were very small (~50-400)bp ...

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... am able to convert 50 mL of chlorella into 60L of the culture. But this is not the main purpose of this project. The main purpose is the algal oil extraction. Both these algae are said to have like 20-25% of lipids in it. I would be extracting that using soxhlet apparatus. But I am stuck at a very ...

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... uses even in E. coli (a species that has no clue about glycosylation whatsoever...) Another problem you may encounter with plants is protein extraction: it is much more laborous to isolate your specific proteins from plant mass than it is from neatly secreted products that hybridoma cells ...

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