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Dissolve

Dissolve

to change or cause to change from a solid to a dispersed form by immersion in a fluid of suitable properties.

Origin: L. Dis-solvo, pp. -solutus, to loose asunder, to dissolve


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Prep. of 50X TAE buffer

No when you are preparing the 0,5M EDTA you first add 80ml of MQwater to the powder, and then try to dissolve the EDTA (it is a long and painful process, but it will eventually happen) by adding NaOH to the solution. And when all the powder has finally dissolved, you just ...

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by canalon
Mon May 04, 2009 1:47 am
 
Forum: Molecular Biology
Topic: Prep. of 50X TAE buffer
Replies: 7
Views: 2034

Prep. of 50X TAE buffer

EDTA is annoying to dissolve to say the least. It is slightly acidic, but disssolves only at pH 8. Also I usually do not prepare my solution in PBS but in water. So take your PH meter, weigh your EDTA and add it to 80ml MQwater, then ...

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by canalon
Sun May 03, 2009 12:20 am
 
Forum: Molecular Biology
Topic: Prep. of 50X TAE buffer
Replies: 7
Views: 2034

Prep. of 50X TAE buffer

... 242g Tris 100mL of 0.5M EDTA: This is prepared by (In 100 ml 1X PBS, Add 73.2 gram of EDTA) 57.1mL Glacial Acetic Acid Add enough MQ H2O to dissolve solids to final volume of 1000mL. Is my work right??? The pH of PBS is about 7.6, Is this Ok for TAE buffer or I have to get the pH to 8??? ...

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by roniadam
Sat May 02, 2009 9:29 am
 
Forum: Molecular Biology
Topic: Prep. of 50X TAE buffer
Replies: 7
Views: 2034

Re: mechanisms of membrane transport

... through the cell membrane for diffusion there is also lots of ways , you may like to elaborate like describe how lipid soluble molecules would dissolve through the lipid bilayer of the membrane. And how small molecules like urea , water and carbon dioxide sqeeze through the phospholipid heads ...

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by sapphirearth
Fri Mar 13, 2009 5:28 am
 
Forum: Molecular Biology
Topic: mechanisms of membrane transport
Replies: 2
Views: 881

Why the pH value decreased seriously in my algal culture?

... solutions: 2X Filner’s Beijernicks Solution (500 ml) NH4Cl 8g CaCl2 * 2H2O 1g MgSO4 *7H2O 2g Trace Mineral Solution (500 ml) 5 g disodium EDTA – dissolve in 400 ml water by heating and stirring Neutralize to pH 6.5 with 5N NaoH Add each of the following in order. Allow each to dissolve completely ...

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by onlinebunny
Wed Mar 04, 2009 11:19 am
 
Forum: Microbiology
Topic: Why the pH value decreased seriously in my algal culture?
Replies: 1
Views: 542
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