Detergent

... in this 2D buffer is 50mM. Do you think it will interfere with the Lowry assay? 3)I assume I can't use Bradford assay here since I am using a detergent, CHAPS. Thanks a lot for your help.

See entire post

I'd try detergent. The pigments you are after are the mainly phycobiliproteins and the chlorophyll. http://en.wikipedia.org/wiki/Phycobilin

See entire post

My class did a simple DNA extraction from cheek epithelial cells. The protocol involved lysing the cells with 4% detergent, 1% NaCl and vortexing, centrifuging at 11krpm to form a cell pellet, and adding cold ethanol to the supernatant to precipitate the DNA. Afterwards we were ...

See entire post

... is used in Polyacrylamide gel electrophoresis, but I'm still having a little difficulty understanding what it does. As far as I can tell, its a detergent that not only breaks up a protein into polypeptides and gives an overall negative charge to the polypeptides. If I remember correctly, gel ...

See entire post

... It is thought to work by damaging the membrane of each viral particle, either by punching holes which cause it to fall apart, or through a "detergent effect" which breaks apart fatty molecules in the membrane. Research leader Dr Scott Van-Compernolle said: "There are lots of substances ...

See entire post