Curves

... Here is a blog post that goes into the 5-parameter logistic in more detail . MasterPlex ReaderFit is specifically designed for drawing standard curves and curve-fitting of unknown points from elisa assays. Allen MiraiBio

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... could in the middle of the theory. That right there explains what would have been going on at the molecular level of the genome that predicts such curves, exactly. Saying something like that happened is no help at all explaining how it works, to begin with. Evolutionary Theory can easily be interpreted ...

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Does anyone know if the numbers of cells at the same OD600s are different between different Escherichia coli strains (when they are grown under the same conditions), or are they same? I would think that the relationship between OD600 and numbers of cells would depend on cell size... Is that correct?...

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... to find the "y". This will give you an estimate for the log(MW) without having to plot anything. This is the way you use calibration curves in general.

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... another 3 sets of 6 (there were 7 as it happens but ignore the first ones, they arnt anything) these two bits of data are put into the standard curves in exactly the same way we did the spot test. secondly there are 3 s and 3 c on each. the 3 s are replicates using protein / enzyme isolates ...

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