Count

... expose the RNA to light too much? It's possible you denatured them. Also, there may have been RNAses in your extract that could have reduced copy count drastically.

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Also, would I use a dilution factor when doing a cell count for a concentrated sample?

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As in the Punnett Square that is the first paper, it is the word balanced that you should take into account for meiosis. This reminds me of the X chromosome, in which to be balanced in the female, one chromosome needs to be inactivated (into a Barr body), and thus already ...

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I use hemocytometer every week! :P Though I wish we bough an automated cell counter, but hey, as long as humans can do it (seemingly) for free, why spend money on things?! Anyway, yeah the damn thing has a high error rate on both low and high concentrations (and ...

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... the thing has an incredibly high error rate anyway (which is why biologists don't really use the thing anymore, there are special devices that can count cells much more accurately).

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