Colonies

... of interest getting transcribed without reporters cause usually you're segretating things afterwards based on the reporters (like trying to grow colonies of bacteria based on resistances and such) so if you start with the gene of interest and then the reporter, you won't have incorrect cells ...

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... in by the polymerase of the bacterium after you transform your ligation mix. Oftentimes i'll get a whole bunch of re-ligated vector when I pick my colonies and sequence the minipreps, so then I go back and CIP the vector and it usually helps. Unfortunately sticky ends are not as foolproof as textbooks ...

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... digested the vector- pcDNA 3.1. Then I ligated the deletion constructs in 4 seperate reactions with the vector pCDNA, picked the transformation colonies, grew, extracted the plasmid, and digested a small amount of the plasmid with the same enzymes to make sure there was correct insert. After ...

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... digested the vector- pcDNA 3.1. Then I ligated the deletion constructs in 4 seperate reactions with the vector pCDNA, picked the transformation colonies, grew, extracted the plasmid, and digested a small amount of the plasmid with the same enzymes to make sure there was correct insert. After ...

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... food bacteria, blue-green algae, and spirochetes Protista large, single eukaryotic cell (nucleus is enclosed by a membrane); some form chains or colonies absorb, ingest, and/or photosynthesize food protozoans and algae of various types Fungi multicellular filamentous form with specialized eukaryotic ...

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