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Coculture

Coculture

(Science: cell biology) growth of distinct cell types in a combined culture. In order to get some cells to grow at low (clonal) density it is sometimes helpful to grow them together with a feeder layer of macrophages or irradiated cells.

The mixing of different cell types in culture is otherwise normally avoided, although it is possible that this could prove an informative approach to modelling interactions in vivo.


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In Vitro Generation of Lymphocytes from ES cells

... trying to generate B cells from murine embryonic stem (ES) cells on OP9 stromal cells. The ES cells look fine and grow nicely, at day 5 of ES/OP9 coculture I see mesoderm-like colonies. But after the passage of nonadherent cells onto fresh OP9 cells (which is necessary at day 5 according to the ...

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by Doro
Tue May 08, 2007 2:51 pm
 
Forum: Cell Biology
Topic: In Vitro Generation of Lymphocytes from ES cells
Replies: 0
Views: 418

asking a question about contamination

hi i want to ask one thing i am diong coculture expt in tissue culture lab and not getting result due to contamination suggest some method to get my results i did my steriisation thourughly,by autoclaving and wiping with ethanol but i don't ...

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by adithya_05
Fri Mar 10, 2006 4:57 am
 
Forum: General Discussion
Topic: asking a question about contamination
Replies: 1
Views: 504


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