Home Forum Dictionary Articles Tutorials Books Directory Share your work


Edit page | Page history | Printable version

Dictionary » C » Cleavage site

Cleavage site

Definition

noun, plural: restriction sites

The site in a polynucleotide chain as to where the restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


Supplement

The restriction enzyme recognizes the site as to where it cuts via a specific sequence of nucleotides along the polynucleotide chain. For example, when the restriction enzyme, Eco RI, recognizes the palindromic sequence GAATTC, it cuts between G and A on the top and bottom strands.


Synonyms:


See also: restriction enzyme


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Question regarding Restriction Enzymes

... presented is shown below: A restriction fragment produced by Bgl II cleavage of Wulfruna wanderensis DNA is cloned into the Bam HI site in pATHB1. When attempting to recover this important piece of DNA from the plasmid why ...

See entire post
by Methtical
Fri Dec 19, 2008 2:01 pm
 
Forum: Genetics
Topic: Question regarding Restriction Enzymes
Replies: 5
Views: 1594

Syntaxin 3

... But I think he also mentioned the RanBP5. So far I know there is only a cleavage site in syntaxin 3 which is missing in the other syntaxins. I searched for this information ...

See entire post
by forsio
Thu Jul 17, 2008 1:06 am
 
Forum: Cell Biology
Topic: Syntaxin 3
Replies: 1
Views: 577

Re: problem with cloning/restriction digest

... or at least 6 extra bases to be able to efficiently cut an NdeI site near the end of a linear fragment. XmaI isn't nearly so sensitive to ... >98%. This is coming from the appendicies in the NEB catalog, "Cleavage Close to the End of DNA Fragments". If your pcr primers don't ...

See entire post
by blcr11
Mon Feb 25, 2008 3:58 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 9042

Zymography Question

... So the molecular weight is either 57 or 45 Kda. By auto proteolytic cleavage the enzyme is activated during the incubation with developing buffer. ... biochemical change (such as a hydrolysis reaction revealing the active site, or changing the configuration to reveal the active site) for it to ...

See entire post
by karthik38
Thu Oct 04, 2007 10:20 am
 
Forum: Bioinformatics
Topic: Zymography Question
Replies: 1
Views: 1752

sanger sequencing

... based on chemical modification of DNA followed by its subsequent cleavage at specific bases. Although this is contemporaneous with the development ... fragments is generated, from the radiolabelled end to the first 'cut' site in each molecule. The fragments are then size-separated in a gel, with ...

See entire post
by CyLMH
Mon Mar 19, 2007 1:50 pm
 
Forum: Molecular Biology
Topic: sanger sequencing
Replies: 2
Views: 1001
View all matching forum results

This page was last modified 09:09, 3 February 2009. This page has been accessed 421 times. 
What links here | Related changes | Permanent link