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Callisons fluid

a diluting fluid for counting red blood cells, consisting of 1 ml of Loeffler's alkaline methylene blue, 1 ml of formalin, 10 ml of glycerol, 1 g of neutral ammonium oxalate, and 2.5 g of sodium chloride added to 90 ml of distilled water, mixed well, and permitted to stand until the solids are dissolved and the reagent is clear; the preparation is filtered prior to use.