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Dictionary » B » Blunt

Blunt

Blunt

1. Having a thick edge or point, as an instrument; dull; not sharp. The murderous knife was dull and blunt. (Shak)

2. Dull in understanding; slow of discernment; stupid; opposed to acute. His wits are not so blunt. (Shak)

3. Abrupt in address; plain; unceremonious; wanting the forms of civility; rough in manners or speech. Hiding his bitter jests in blunt behavior. A plain, blunt man.

4. Hard to impress or penetrate. I find my heart hardened and blunt to new impressions. (Pope)

blunt is much used in composition, as blunt-edged, blunt-sighted, blunt-spoken.

Synonym: obtuse, dull, pointless, curt, short, coarse, rude, brusque, impolite, uncivil.

Origin: cf. Prov. G. Bludde a dull or blunt knife, dan. Blunde to sleep, Sw. & Icel. Blunda; or perh. Akin to E. Blind.


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Re: Ligation and expression trouble

... producing overhangs of different bases how can the vector religate, in other words how would the CIP help, wouldnt using CIP make more sense in blunt end producing enzymes? I had followed points 4 and 6. Thanks for -Point 5 its interesting, I am gonna look into that! Wow I have never gone above ...

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by solution
Wed Jul 08, 2009 9:16 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

pcDNA 4/TO

... this but I think it would have been better if you had ends which are not complementary on the plasmid to prevent it from closing on itself. Maybe blunting one end of the plasmid and the corresponding end on the insert and using a ligase which connects blunt ends. Also are you sure you are getting ...

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by GreenDog
Tue Jan 13, 2009 10:56 am
 
Forum: Molecular Biology
Topic: pcDNA 4/TO
Replies: 1
Views: 458

Quick Question !

... marked with different colors to the two kinds of cDNA. Never heard of an adaptor before. I think it may have been used to talk about converting blunt-end restriction sites into stick-end'ed restriction sites.. but that has nothing to do with the next part, hybridization. Hybridize, remove hybridized ...

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by Jonesy
Wed Dec 31, 2008 2:53 pm
 
Forum: Genetics
Topic: Quick Question !
Replies: 7
Views: 617

Help please! Directional cloning: colonies with WEIRD DNA?

Hello all, I desperately need help with molecular cloning (directional, not blunt end). I've been going at this for 5 months, and I believe if I can overcome this hurdle I will be good to go. Please help, and thanks in advance! Debriefing: Basically, I have a ...

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by walkthewok
Sun Nov 30, 2008 5:41 am
 
Forum: Molecular Biology
Topic: Help please! Directional cloning: colonies with WEIRD DNA?
Replies: 1
Views: 550

problem with cloning using pTYB2

... guess would be that the pcr product didn't digest with both enzymes--but that is just a guess. Your ligation is going to be a mixed sticky-end and blunt-end ligation. You could try doing a short (2-8 hr) ligation at 30 ul (which I guess is already more dilute than the "standard" ligation ...

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by blcr11
Tue May 06, 2008 5:08 pm
 
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 1303
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