Dictionary » B » Blot

Blot

Blot

1. To spot, stain, or bespatter, as with ink. The brief was writ and blotted all with gore. (Gascoigne)

2. To impair; to damage; to mar; to soil. It blots thy beauty, as frosts do bite the meads. (Shak)

3. To stain with infamy; to disgrace. Blot not thy innocence with guiltless blood. (Rowe)

4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. One act like this blots out a thousand crimes. (Dryden)

5. To obscure; to eclipse; to shadow. He sung how earth blots the moons gilded wane. (Cowley)

6. To dry, as writing, with blotting paper.

Synonym: to obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch.

Origin: cf. Dan. Plette. See blot.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Problems with gene knockdown

... infected cells (in both rat and mouse cell lines) were selected on antibiotic. But when I look at the protein expression in the cells by Western blot, there seem to be no reduction. Of the sequences, at least 3 showed significant knockdown in Luciferase assay. Does anyone know any other way to ...

See entire post
by zodiac1505
Tue Sep 08, 2009 11:31 am
 
Forum: Molecular Biology
Topic: Problems with gene knockdown
Replies: 0
Views: 68

Re: northern blot analysis

hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help... i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting ...

See entire post
by robsabba
Thu Aug 27, 2009 7:25 pm
 
Forum: Molecular Biology
Topic: northern blot analysis
Replies: 2
Views: 160

Western Blot from an Agarose gel...?

... a 4% agarose gel for protein separation using 1x tgs buffer. I was wondering if it would be pointless (or even detrimental) to perform a Western blot on the same gel, just to visualize it better. help? Thanks

See entire post
by herm913
Thu Aug 13, 2009 12:56 pm
 
Forum: General Discussion
Topic: Western Blot from an Agarose gel...?
Replies: 3
Views: 111

northern blot analysis

hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help... i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting ...

See entire post
by proteinz
Sat Aug 01, 2009 12:14 pm
 
Forum: Molecular Biology
Topic: northern blot analysis
Replies: 2
Views: 160

DNA hybridisation

Can anyone help me here Iam struggling to understand how to calculate the amount of DNA probe i need for my hybridization solution in a blot. say for a hybridization solution of 450 microliter, I have 0.5pmol DNA probe, what volume of the probe i need for this hybridization solution?

See entire post
by bizy
Tue Jun 30, 2009 6:00 am
 
Forum: Molecular Biology
Topic: DNA hybridisation
Replies: 0
Views: 125
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 807 times. 
What links here | Related changes | Permanent link