Beads

Hi there, We did an experiemnt the other day where we had to sep. out two proteins via a colum containing CM beads. It had a pH of 6 and both proteins bound to this. So to sep them out we used a pH of 7.6 and collected a sample and then used a pH and of 9 and collected sample ...

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... comment and give suggestions. PCR Tube- Mixed inside PCR tube 10ul of 1% SDS solution 10ul of Protein K Kinase 15ul of Nucleas free water. 15 Beads from a Zippy Fecal Extraction kit, Then Vortexted at the highestl level for 30 minutes. PCR tube then Placed in a Boiling water bath for 15 miniutes ...

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I have question about the steps involved in SAGE. So, the mRNA is bound to beads, converted to cDNA and then it's cleaved. For the following steps, are we working with the part of the cDNA that's attached to the bead? or are we wroking with the released piece ...

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there are a number of different experiments you could do to analyze this. Beads are probably the best and the easiest, but you could also do, for example, an electrophoretic mobility shift assay. But you see that I am trying to find whether my protein ever binds ...

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specific? a bead that binds dna of course So if my protein is holding a piece of DNA, it will bind beads that has an affinity to DNA. And use antibodies to detect it on Western maybe? Do you have any literature I can look up to get more details?

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