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Banding patterns

Banding pattern

(Science: genetics) chromosomes stained with certain dyes, commonly quinacrine (Q banding) or giemsa (g banding), show a pattern of transverse bands of light and heavy staining that is characteristic for the individual chromosome.

The basis of the differential staining, which is the same in most tissues, is not understood: each band represents 5-10% of the length, about 10exp7 base pairs, although this is not true for polytene chromosomes in drosophila that show more than 4000 bands.


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Re: protein purification Ni-NTA

... also try different IMAC resins to see if there are differences in the banding patterns, again suggesting that they could be non-specifically bound contaminants.

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by blcr11
Wed Apr 16, 2008 6:40 pm
 
Forum: Molecular Biology
Topic: protein purification Ni-NTA
Replies: 1
Views: 649

Southern Blotting II

... I have attached a picture of the membrane. Can you explain why the banding patterns are different in the three different lanes? file:///C:/Documents%20and%20Settings/Pooja%20Manjunath/Desktop/Southern%20Blotting%20II.bmp

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by Agronomy
Tue Mar 20, 2007 6:52 pm
 
Forum: Genetics
Topic: Genetics Question
Replies: 7
Views: 1051

Genetics Question

... I have attached a picture of the membrane. Can you explain why the banding patterns are different in the three different lanes?

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by Agronomy
Tue Mar 20, 2007 6:47 pm
 
Forum: Genetics
Topic: Genetics Question
Replies: 7
Views: 1051

Genetics Question

... no mistake or technical problems in the Southern transfer, explain why banding patterns are not the same in lanes 1, 2, and 3. I am really lost on this question? Any help ...

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by Agronomy
Tue Mar 06, 2007 11:44 pm
 
Forum: Genetics
Topic: Genetics Question
Replies: 7
Views: 1051

Banding patterns on chromosomes

Ok, so how I understand it is... the banding patterns don't indicate gene activity, but are used to distinguish chromosomes... but patterns can indicate ...

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by imraan47
Thu Oct 26, 2006 8:41 pm
 
Forum: Molecular Biology
Topic: Banding patterns on chromosomes
Replies: 3
Views: 639
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