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Ashby method

a differential agglutination method for estimating erythrocyte life span; compatible blood possessing a group factor that the recipient lacks is transferred to the recipient; after the transfusion, sera with potent agglutinins for the recipients red cells are added to samples of the recipients blood, and the unagglutinated red cells are counted; using this technique the red cell life span in normal persons is found to be 110 to 120 days.