Aseptic

BUMP

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I have personally never used anti-fungal drugs in my cultures; good aseptic technique is well enough to keep your cultures clean. Unlike anti-microbial drugs, anti-fungal ones target eukaryotic cells, and thus the chances of side effects are much bigger with ...

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Neither yeast (spores are everywhere) nor mycoplasma (you are probably carrying some on your hands right now) don't care squat about antibiotics.

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why is it important in cell culture to work under aseptic conditions when the growth medium already contains antibiotics? Any ideas?

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Buy new toilet rolls, they should be safe. Not aseptic, but probably no more (and probably less) contaminated than anything around. Unless you are working with some Bubble children, without any immune system this is just a waste of time. Or are ...

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