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Antisense strand

Antisense strand

(Science: molecular biology) The strand of dna which is not used during transcription to make mrna (anticoding strand). The mrna made during transcription thus has the same sequence as this strand, so that the eventual protein will be a sense version.


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Re: Help with sequencing DNA.

For this mRNA: AAGGGUGUAGAAGCUAAGGGU The antisense is likely: ACCCTTAGCTTCTACACCCTT The sense is likely: AAGGGTGTAGAAGCTAAGGGT ... be a pre-mRNA splicing event within the sequence that produces the mature mRNA strand. If so, the DNA sequence would be interrupted by an intron. If there is no ...

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by jonmoulton
Mon Jun 29, 2009 4:47 pm
 
Forum: Molecular Biology
Topic: Help with sequencing DNA.
Replies: 3
Views: 189

Re: How to knock down a gene?

... an oligo into cells, such as one of the following: RNase-H competent antisense -- ssDNA oligos ssRNA oligos Phosphorothioate oligos Many sorts ... RNA that are recognized by the enzyme RNase-H, which cleaves the RNA strand. RNase-independent oligos bind to the mRNA and get in the way of processes; ...

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by jonmoulton
Fri Oct 31, 2008 6:46 pm
 
Forum: Genetics
Topic: How to knock down a gene?
Replies: 1
Views: 805

DNA Transcription

... questions: The following DNA sequence is found in a segment of double-stranded DNA that encodes for the English word describing protein that is ... word for this protein? Be sure to note the 5' and 3' ends. What is the antisense DNA sequence that would be found in the template strand for the ...

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by randylo
Thu May 15, 2008 3:32 pm
 
Forum: Molecular Biology
Topic: DNA Transcription
Replies: 4
Views: 1520

How would you create a strand specific PCR?

Hi! I want to run a strandspecific PCR. I have sense and antisense RNA transcript as control. How would you do it? The simpliest would be just to use one primer for ...

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by Ammie
Fri Apr 18, 2008 1:52 pm
 
Forum: Molecular Biology
Topic: How would you create a strand specific PCR?
Replies: 0
Views: 629

sense and antisense

... polyA tail, I turn that into cDNA...is the newly made cDNA the sense or antisense strand?...man I have found definitions (inc. this site) of the meaning of these 2 strands but ...

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by biology_06er
Sun Apr 06, 2008 5:07 am
 
Forum: General Discussion
Topic: sense and antisense
Replies: 1
Views: 423
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