Anion

... solution for the column is 20mM TRIS(pH 8.5)... and I salt out using KCl across the concentration gradient of 0.1M - 1.0M... and the column is a anion exchanger, sepharose (Q). Now the problem is I'm not seeing my protein after elution, roughly about 20 times the volume of the column has been ...

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... protein. Penicillinase (bla) from E. coli has a theoretical pI of 9.4, according to ProtParam on the Expasy proteomics server. Q-type resins are anion exchangers (that is, they bind negatively charged species). To get lactamase (at least bla from E. coli) to be negatively charged, you need to ...

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... of uracil induced by LEEs, we theoretically characterized the potential energy surfaces (PESs) along the N-H and C-H bonds of the uracil anion, as well as the energetics involved. The PESs show that, activation barrier of less than 1 eV exists for the N1-H dissociation with rather flat ...

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... the mid-19th century, inorganic layered double hydroxide (LDH) ceramics are used for a variety of applications including antacids, catalysts, anion exchangers, and adsorbents. The intercalated anion, within the interlayer space between the cation hydroxide layers, can be readily exchanged ...

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... limited iron ion pool decreases the possibility of Fenton reaction and the formation of extremely reactive hydroxylradical from superoxide radical anion and hydrogen peroxide (Pilas et al., 1988). It has also been shown that melanin imitates action of superoxide dismutase(SOD), thus additionally ...

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