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Adsorption

Adsorption

1. (Science: chemistry) The accumulation or concentration of molecules of a gas or liquid on a surface in contact with the gas or liquid, resulting in a relatively high concentration of the gas or solution at the surface. Note that the accumulating molecules do not actually penetrate the substance they are on.

Compare: absorption.

2. (Science: virology) The adhesion of a substance to an organic particle in a solution, for example the adhesion of a virus to a cell.

Origin: L. Sorbere = to suck


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Is an ELISA plate really specific for protein adsorption?

Not lipids, DNAs, etc. ? What about lipoproteins and glycoproteins? Could anybody provide some basis for this specific interactions of protein molecules w/ surface groups at the bottom of the ELISA plate wells? Or links to some orginal research papers? Thanks.

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by hanhan2008
Tue Dec 04, 2007 3:39 pm
 
Forum: Molecular Biology
Topic: Is an ELISA plate really specific for protein adsorption?
Replies: 7
Views: 971

Folin-Wu Protein Precipitation method

... concentration of (NH4)2SO4 precipitates globulins and not albumin from a serum? and what component of a serum is affected by coprecipitation and adsorption? is it just the protein component? THANKS A MILLION!!!

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by darkchoc
Tue Mar 13, 2007 6:05 am
 
Forum: Molecular Biology
Topic: Folin-Wu Protein Precipitation method
Replies: 4
Views: 2287

Protein precipitation

... other acidic barium are used to precipitate albumin instead of to recover it? Barium will attach and cover albumin molecules surface (yes, it is adsorption), so that it cannot dissolve/being precipitated? :?

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by Dr.Stein
Tue Mar 06, 2007 8:00 am
 
Forum: Molecular Biology
Topic: Protein precipitation
Replies: 4
Views: 1290

Protein precipitation

... "Barium hydroxide would be a possible method to recover precipitated proteins because there would be a chemical reaction with the proteins as aDsorption would occur on to the precipitate" aDsorption means that the there is attachment to the surface unlike aBsorption involving it to be ...

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by Miss_Me
Mon Mar 05, 2007 11:03 am
 
Forum: Molecular Biology
Topic: Protein precipitation
Replies: 4
Views: 1290

Molar Extinction Coefficient

... the extinction coefficient- if you can find it check out Protein Science (1995) 4:2411-2423; Pace et al "How to measure and predict the molar adsorption coefficient of a protein". Good luck!

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by cbourne
Tue Jan 30, 2007 7:55 pm
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient
Replies: 4
Views: 15787
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