Adherent

Hi I am going to establish single cell clones from adherent and suspend CHO cells, my colleques said that it isnt so easy do you had any expereince it that?

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... is ~1ug of purified 6x his-tagged soluble (and correctly processed) protein. The c-DNA in question is about 4k bp. I'm hoping to get away with an adherent culture, since we haven't got a special twirly incubator. Heck, I'm not even sure a baculovirus is what I want, but I think it's probably the ...

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... marker to verify the BT-20, I only observed the morphology of the BT-20 cells, and checked if they are attached to the plates, as they are adherent cells. Sorry, I don`t know what do you mean: how does that look on a graph?

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... re-plated and allowed to grow back to confluency (or to some maximum density if you're using suspension cultures). Each time you trypsinize an adherent cell line to replate it, you add 1 to the passage number. The higher the passage number, the farther away you must be from the primary explant--or ...

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... cells from one cell is not quite the same thing as trying to get a karyotype from a single cell. Which is it you want to do? If you are growing adherent cells like fibroblasts, just titrate them out at low density. Wait a few days and then isolate individual "colonies" of cells. Use ...

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