Acetic

... solutions and powders available: NaCl powder (molecular weight 58.4), 1 M potassium phosphate, and a 5 mg/ml stock of cholesterol (mol. wt. 386.7) Acetic acid (CH3COOH) is a liquid at room temperature. When making acetate buffers (as in the question above) you must add specific molar quantities ...

See entire post

... i can give you some general advice. In this case you have to take in consideration not only the pH of the PBS solution but also the effect of the acetic acid (which will be "pulling" your pH down into acidic) so if yout pH is to low try lowering the pH by adding a base such as NaOH

See entire post

... To make 1L of 50x TAE buffer: 242g Tris 100mL of 0.5M EDTA: This is prepared by (In 100 ml 1X PBS, Add 73.2 gram of EDTA) 57.1mL Glacial Acetic Acid Add enough MQ H2O to dissolve solids to final volume of 1000mL. Is my work right??? The pH of PBS is about 7.6, Is this Ok for TAE buffer ...

See entire post

... Filner’s Beijernicks Solution 25ml 1M Potassium Phosphate 1ml Trace mineral solution or 5ml P IV solution 2X (see SVM) 3ml Tris-Base 2.42g Glacial Acetic Acid (17.4 mM acetate) 1ml (pH should be 7.2) Stock solutions: 2X Filner’s Beijernicks Solution (500 ml) NH4Cl 8g CaCl2 * 2H2O 1g MgSO4 *7H2O ...

See entire post

... Filner’s Beijernicks Solution 25ml 1M Potassium Phosphate 1ml Trace mineral solution or 5ml P IV solution 2X (see SVM) 3ml Tris-Base 2.42g Glacial Acetic Acid (17.4 mM acetate) 1ml (pH should be 7.2) Stock solutions: 2X Filner’s Beijernicks Solution (500 ml) NH4Cl 8g CaCl2 * 2H2O 1g MgSO4 *7H2O ...

See entire post